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From what I've read in the manual, yes you should be able to use this with Mobius without a sound card. In addition to being a mixer, it is also a 2 in 2 out USB audio interface. The difference between this and most other multi-channel audio interfaces is that you can only send to the computer a stereo mix of all of the mixer inputs. The Firebox for example has 6 inputs that are individually sent to the computer. The Mackie is nice if you prefer a hardware mixer and don't need to insert software effects on individual channels. With the Firebox you wold use a software mixer which is more flexible, but you have to deal with a mousey user interface. The Firebox also has 6 outputs so you could set up a surround output mix from the computer. The Mackie only has a single stereo pair of output channels from the computer. As far as latency, it all depends on how good Mackie is at writing device drivers. Since this is only a 2x2 interface I would expect latency to be as good or better than anything else out there. You don't need firewire for a 2x2, USB is fine. But this is relatively new and Mackie doesn't have a long history with audio interfaces, so I would check the forums to see if anyone is having problems. Jeff